A rapid fluorometric assay of angiotensin-converting enzyme.

Angiotensin Converting Enzyme in Sarcoidosis

Fluorometric assay for angiotensin converting enzyme in human serum by centrifugal analysis.

Due to its action on angiotensin I and bradykinin, angiotensin converting enzyme (ACE, dipeptidyl carboxypeptidase, EC 3.4.15.1) plays a crucial role in blood pressure regulation [l]. Attention was focused on this enzyme when Lieberman observed elevated serum ACE levels in active sarcoidosis [2]. Because ACE is a sensitive index both in assessing the clinical status of this disease and in monit...

Inhibitor binding assay for angiotensin-converting enzyme.

We describe a new principle for the determination of enzymes, here applied to angiotensin-converting enzyme (ACE, EC 3.4.15.1) in human serum. The enzyme inhibitor binding assay is based on specific binding of labeled inhibitor to the active center of the enzyme. Serum (10-15 microL) is incubated with 125I-labeled ACE inhibitor (" 351A ," a p- hydroxybenzamidine derivative of N-(1-carboxy-3-phe...

Characterization of a spectrophotometric assay for angiotensin converting enzyme.

Although serum angiotensin converting enzyme (EC 3.4.15.1) activity has generally been shown to be increased in patients with sarcoidosis, considerable variation in the diagnostic usefulness of this test has been reported. We investigated the possibility that this variation may be the result of inhibition of the widely used spectrophotometric assay by various substances. We also prospectively e...

A rapid assay for angiotensin-converting enzyme activity using ultra-performance liquid chromatography-mass spectrometry.

Angiotensin-converting enzyme (ACE) plays an important role in the renin-angiotensin system and ACE activity is usually assayed in vitro by monitoring the transformation from a substrate to the product catalyzed by ACE. A rapid and sensitive analysis method or ACE activity by quantifying simultaneously the substrate hippuryl-histidyl-leucine and its product hippuric acid using an ultra-performa...